Exploring the efficacy of non-invasive 40Hz tACS stimulation in AD mice

Project objectives

Neuronal hyperexcitability and epilepsy contribute to Alzheimer's disease (AD) pathology by increasing amyloid-β (Aβ) burden. These defects correlate with reduced γ-oscillations, suggesting impaired neuronal function of parvalbumin and a causal link between reduced γ-waves, brain hyperexcitability, and Aβ load. Indeed, restoration of γ oscillations with 40 Hz stimulation in AD mice reduces Aβ, inflammation, cell loss, and cognitive decline. However, clinical trials with 40 Hz pacing in patients (NCT03290326, NCT03880240, NCT04055376) are still ongoing and are primarily focused on Aβ burden. It is not yet known whether γ stimulation improves synaptic defects and neuropsychiatric symptoms. This project proposes to test non-invasive transcranial alternating current stimulation (tACS) at a frequency of 40 Hz to obtain preclinical data in a mouse model of AD. The aim of the project is to evaluate whether repetitive sessions of 40Hz tACS are able to improve behavioral and synaptic deficits in AD mice.

Start and end date

August 2022 - July 2025

Project Manager

Dr. Paraskevi Krashia, Coordinator

Coordinating institution of the project

Università Campus Bio-Medico di Roma

Other Institutions involved

IRCCS Santa Lucia Foundation, Rome

Funding source

Alzheimer's Association (USA)

Economic value of the project

$ 159.000