PON1_01602 BioKer

Project objectives

Within the PON01_01602 Project and OR 2.2, the activity of the Molecular Medicine and Biotechnology Laboratory is included in ARI 2.2.4 - Study and development of diagnostic protocols. One or more of the following methods are being studied and developed for the identification of the OOF protein in biological samples:

• Absolute qRT-PCR method for the qualitative and quantitative identification of OOF protein in biological samples consisting of human tumor cells positive for OOF antigen

• FACS method based on the use of the anti-OOF antibody fragment to identify leukemic cells expressing the marker constituted by the bcr/abl-OOF fusion protein directly in blood samples taken from Philadelphia-positive leukemic patients.

In this phase, the protocols studied in the previous research phases are also validated with the aim of developing an analytical methodology applicable to the quantitative identification by FACS analysis of leukemic cells expressing the bcr-abl-OOF fusion protein and/or an analytical kit for the quantitative determination of BCR-ABL-OOF transcripts in total RNA extracted from the blood of leukemic patients using real time.

Start and end date

01/10/2011 - 31/10/2015

Project Manager

Prof. Vito Michele Fazio - Scientific manager

Coordinating institution of the project

Bioker srl

Other Institutions involved

• University of Naples - Pharmacology Division, Department of Neuroscience, Department of Endocrinology and Oncology (CIRPEB)

• IRCCS House for the Relief of Suffering, San Giovanni Round (FG)

Funding source(s).

Ministry of Education, University and Research