Prevalence study to assess interaction between the Gut Microbiome and the immune system in the pathogenesis of inflammatory bowel diseases, such as IBD and IBS
Project objectivesThe study's hypothesis is to verify whether there is a correlation between inflammatory bowel diseases (IBD and IBS) and specific changes in the composition of the fecal or intestinal mucosa-associated microbiome. To this end, the composition of the intestinal microbiome will be studied using Next Generation Sequencing (NGS) methods, followed by high-resolution phylogenetic analysis to identify specific relationships between the identified microbiome type and the severity of inflammatory bowel disease. Furthermore, the presence of specific polymorphisms in genes involved in innate immunity, such as NOD2 and ATG16L1, already associated with the development of Crohn's disease, will be analyzed to determine whether the presence of these polymorphisms is associated with a modification of the intestinal microbiome, thus predisposing to the development or progression of the disease. Finally, the activity of the enzyme diamine oxidase (DAO), a biochemical marker of inflammation, will also be assessed in both intestinal biopsy extracts and in the serum of patients compared to healthy subjects. |
Start and end date |
|
09/11/2014 - 09/11/2017 |
Project Manager |
|
Dr Michele Guarino |
Coordinating institution of the project |
|
Istituto Superiore di Sanita |
