EoAG - Flow cytometric analysis of eosinophilic activity mediated by the expression of cell membrane markers in subjects with severe asthma: baseline characterization and verification of changes induced by therapy with biological drugs

Project objectives

Primary Objective:

• To describe the expression of membrane molecules related to eosinophil activation status (phenotype) in asthma patients and determine its predictive role in response to biological treatment. We will also test whether a particular phenotype can be used as a molecular signature of an asthma endotype.

Secondary Objectives:

• To test whether a patient population exhibiting a pro-inflammatory eosinophil phenotype, even with low to normal eosinophil counts, would exhibit clinical features of severe, refractory asthma; 
• To evaluate whether biological treatment is able to determine longitudinal changes in the expression of eosinophil membrane molecules after 12 months of treatment.

Start and end date

Project Manager

Coordinating institution of the project

Other Institutions involved

• Flow Cytometry Laboratory IRCCS SanRaffaele, Val Cannuta, Rome (Head Dr. Laura Vitiello)

Funding source(s).