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ITHACA

Technology Integration High throughput for the screening methods Faster Antibodies for Antitumor Therapies

Project objectives

The ITHACA project is divided into 3 operational work packages (WP) and 1 management work package. Specifically, this project aims to create a technological platform based on microfluidic systems high throughput to identify and select, by means of fluorescent probes, cell clones that produce antibodies specific for the antigen of interest (recombinant or present on the membrane of a tumor cell), to be used for tumor immunotherapy or for diagnostics. This system will be validated in a laboratory environment by means of advanced microscopy methodologies. The developed platform will therefore ensure high compatibility with a variety of laboratory equipment, such as automated manipulators, microscopes and screening systemsing; it will also be able to satisfy different needs such as: cell-cell interaction, simplicity of set-up, reduced use of reagents and achievement of significant results in short times, with low costs. It will represent an efficient alternative to traditional methods.
The therapeutic potential of the identified monoclonal antibodies will then be tested using a model on chip specifically designed to reproduce the tumor-immune system interaction. Understanding the dynamic relationships between the components of the tumor microenvironment is essential for the development of new therapeutic strategies, such as antitumor immunotherapies based on monoclonal antibodies, which can prevent human tumor resistance to treatment.
The ultimate goal will be to integrate the measurement platform for the screening methods high-performance in the company production line.

Start and end date

November 2020 – December 2023

Project Manager

Alberto Rainer

Coordinating institution of the project

Takis Biotech Srl

Other Institutions involved

National Research Council (CNR)

Funding source(s).

POR FESR Lazio 2014-2020 – Lazio Region Public Notice «Strategic Projects 2019»

Link to the project website

https://www.takisbiotech.it/research/14-projects/264-ithaca

 

 

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